Gibson introduced the B-25 in 1961 which featured a mahogany body (solid back, laminated sides), solid spruce top, and rosewood fingerboard like the B-45. The B-25 featured a smaller body than the B-45 and a narrow neck (1 5/8" nut). Many came with a plastic bridge and ceramic adjustable saddle. Most B-25's had a cherry sunburst finish, but there were a limited number of ebony finish editions with white pickwards. There was also a natural finish B-25-N edition. Production of these standard B-25's ended in 1977.

Gibson produced a "short scale" version of the B-25, the B-25-3/4, with the same features as the standard B-25 but with a ¾ size body and a different sunburst finish. Gibson manufactured this edition from 1962 through 1968. From 1966 – 1968, the natural finish B-25-N-3/4 was also available. The short scale editions were marketed as "student" guitars.

Two 12-string editions of the B-25 were also available starting in 1962. The B-25-12 had a cherry sunburst finish and was available through 1970. Gibson manufactured the natural finish B-25-12-N through 1977, the last year of production for all B series guitars.

In 2012, Gibson Custom Shop launched the "Icon '60s" series, reissued many classic models. Although never appeared on Gibson's official website or media presses, there is a batch of B-25 and B-24 3/4 also produced, especially for the Japan market.